Monitoring ascorbic acid oxidation with H2O2 and analyzing the food supplement product using parallel factor analysis model of kinetic UV absorption measurements

DİNÇ E., ÜÇER A., ÜNAL M. N.

JOURNAL OF FOOD COMPOSITION AND ANALYSIS, cilt.123, 2023 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 123
  • Basım Tarihi: 2023
  • Doi Numarası: 10.1016/j.jfca.2023.105600
  • Dergi Adı: JOURNAL OF FOOD COMPOSITION AND ANALYSIS
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, PASCAL, Analytical Abstracts, BIOSIS, Biotechnology Research Abstracts, CAB Abstracts, Food Science & Technology Abstracts, Veterinary Science Database
  • Anahtar Kelimeler: Ascorbic acid oxidation, Kinetic UV absorption measurement, PARAFAC model, Quantification, Vitamin C
  • Ankara Üniversitesi Adresli: Evet

Özet

Ascorbic acid is a functional compound in maintaining the physiological processes of humans who acquire it from dietary sources, and also functions as an antioxidant. Therefore, the quantitation, and degradation of ascorbic acid in the food matrix and its interaction with coexisting components is a very important issue in terms of food safety and human health. Classical analytical methods may not give the expected results in the analysis of more advanced and complex food products obtained with the developing production technology. In this perspective, the parallel factor analysis (PARAFAC) model using kinetic UV absorption measurements was improved for the co-estimation of the kinetics and quantification of ascorbic acid in a food supplement product, which is a commercial effervescent preparation without requiring a prior separation step. The proposed three-way analysis strategy was validated and applied to the analysis of the relevant samples. For example, in the test samples, percent recovery and relative standard deviation were found to be 99.4 % and 1.36 %, respectively. The proposed chemometric strategy would be an alternative approach with a good selectivity for estimating the kinetics and quantification of ascorbic acid in a commercial supplementary product. The assay results of the PARAFAC model was checked by using ultra-performance liquid chromatography (UPLC).