Detection of Escherichia coli O157:H7 in ground beef using immunomagnetic separation and multiplex PCR

SARIMEHMETOĞLU B., Aksoy M. H., Ayaz N. D., Ayaz Y., KÜPLÜLÜ Ö., Kaplan Y. Z.

Food Control, cilt.20, sa.4, ss.357-361, 2009 (SCI-Expanded) identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 20 Sayı: 4
  • Basım Tarihi: 2009
  • Doi Numarası: 10.1016/j.foodcont.2008.06.002
  • Dergi Adı: Food Control
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.357-361
  • Anahtar Kelimeler: E. coli O157:H7, Ground beef, Multiplex PCR
  • Ankara Üniversitesi Adresli: Evet

Özet

In the study 251 fresh ground beef samples sold in Ankara were analyzed to evaluate the prevalence of Escherichia coli O157:H7 by immunomagnetic separation (IMS) based cultivation technique. Virulence factors of the isolates were determined by multiplex PCR. Nineteen (7.6%) of 251 ground beef samples were found as contaminated with E. coli O157. By PCR analyse in two of the isolates fliCh7 gene was detected and identified as E. coli O157:H7. According to the multiplex PCR one of the isolate has all stx1, stx2, eaeA, hly and fliCh7 genes and the other has stx1, eaeA, hly and fliCh7 genes. © 2008 Elsevier Ltd. All rights reserved.