Detection of Escherichia coli O157:H7 in ground beef using immunomagnetic separation and multiplex PCR

SARIMEHMETOĞLU B., Aksoy M. H., Ayaz N. D., Ayaz Y., KÜPLÜLÜ Ö., Kaplan Y. Z.

Food Control, vol.20, no.4, pp.357-361, 2009 (SCI-Expanded) identifier

  • Publication Type: Article / Article
  • Volume: 20 Issue: 4
  • Publication Date: 2009
  • Doi Number: 10.1016/j.foodcont.2008.06.002
  • Journal Name: Food Control
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.357-361
  • Keywords: E. coli O157:H7, Ground beef, Multiplex PCR
  • Ankara University Affiliated: Yes

Abstract

In the study 251 fresh ground beef samples sold in Ankara were analyzed to evaluate the prevalence of Escherichia coli O157:H7 by immunomagnetic separation (IMS) based cultivation technique. Virulence factors of the isolates were determined by multiplex PCR. Nineteen (7.6%) of 251 ground beef samples were found as contaminated with E. coli O157. By PCR analyse in two of the isolates fliCh7 gene was detected and identified as E. coli O157:H7. According to the multiplex PCR one of the isolate has all stx1, stx2, eaeA, hly and fliCh7 genes and the other has stx1, eaeA, hly and fliCh7 genes. © 2008 Elsevier Ltd. All rights reserved.