CHARACTERIZATION OF FLAVONOIDS IN PETALS OF ROSA-DAMASCENA BY HPLC AND SPECTRAL-ANALYSIS

VELIOGLU Y. S., MAZZA G.

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, cilt.39, sa.3, ss.463-467, 1991 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 39 Sayı: 3
  • Basım Tarihi: 1991
  • Doi Numarası: 10.1021/jf00003a007
  • Dergi Adı: JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.463-467
  • Ankara Üniversitesi Adresli: Evet

Özet

High-performance liquid chromatography (HPLC), performed with a photodiode array detector, was applied to separation of the anthocyanins and other flavonoids in the petals of Rosa damascena Mill., used to produce rose petal jam and attar of rose. Over 25 discrete peaks were detected, and separation of these took less than 50 min. Most of the HPLC peaks were collected as separate fractions from the column outlet and reanalyzed by HPLC, paper chromatography, and spectral analysis. The concentration of total anthocyanins was 285 mg/kg of fresh petals. The main anthocyanin was cyanidin 3,5-diglucoside, which accounted for over 95% of the total anthocyanins. Also identified were several kaempferol and quercetin glucosides, galactoside, arabinosides, and rhamnosides.