Metagenomics and volatile profile of Turkish artisanal Tulum cheese microbiota

GEZGİNÇ Y., Karabekmez-Erdem T., Tatar H. D., DAĞGEÇEN E., Ayman S., AKYOL İ.

FOOD BIOSCIENCE, cilt.45, 2022 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 45
  • Basım Tarihi: 2022
  • Doi Numarası: 10.1016/j.fbio.2021.101497
  • Dergi Adı: FOOD BIOSCIENCE
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Food Science & Technology Abstracts, INSPEC
  • Anahtar Kelimeler: Microbiota, 16S rRNA profiling, Metagenomics, Tulum cheese, Volatile profile, LACTIC-ACID BACTERIA, AROMA COMPOUNDS, RAW-MILK, LIPOLYSIS, STARTER, PROTEOLYSIS, DIVERSITY, IDENTIFICATION, EVOLUTION, DYNAMICS
  • Ankara Üniversitesi Adresli: Evet

Özet

Turkish artisanal Tulum cheese is a traditional raw milk cheese and starter cultures are not used for ripening process. In this study, 16S rRNA amplicon-based sequencing approach was used for determining bacterial diversity in Tulum cheeses. The volatile organic compound (VOC) profile which contributes to sensory properties of the cheeses was determined via solid-phase microextraction combined with gas chromatography-mass spectrometry (GC-MS/SPME) and correlated with the microbiota. In Tulum cheese samples, Lactobacillaceae (30,71%) phylum widely varied but Streptococcaceae (59,86%) was dominant. It mainly belongs to the four dominant genera of lactic acid bacteria (LAB) and includes Lactobacillus, Lactococcus, Streptococcus and Pediococcus. The bacterial diversity of TC5 sample was much higher than that of other samples. Moreover, several species of non-starter LAB were frequently detected. A total of 36 volatile compounds were identified including hydrocarbons (3), acids (13), alcohols (5), ketones (3), aldehydes (3), esters (8) and miscellaneous compound (1). Pentadecanoic acid, octadecanoic acid, tetradecanoic acid, octanoic acid, hexanoic acid were determined to be dominant in all cheese samples and to be related to microbial flora. Also, the sensory profiles of the samples were examined. The most popular TC6 did not contain 2-butanone and hexanal from the volatile organic compound (VOC) values, while in the most unpopular TC3, 2-butanone (7.73%) and hexanal (5.95%) were found to be the highest. The outcome of this study will contribute to literature on bacterial diversity and aromatic profile of Tulum cheese which should be investigated in order to improve quality and safety.