The effect of temperature and contact time of sodium hypochlorite on human roots infected with Enterococcus faecalis and Candida albicans
ODONTOLOGY, vol.102, no.1, pp.36-41, 2014 (SCI-Expanded, Scopus)
- Publication Type: Article / Article
- Volume: 102 Issue: 1
- Publication Date: 2014
- Doi Number: 10.1007/s10266-012-0086-x
- Journal Name: ODONTOLOGY
- Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
- Page Numbers: pp.36-41
- Keywords: Sodium hypochlorite, Preheating, C. albicans, E. faecalis, VITRO ANTIMICROBIAL ACTIVITY, IN-VITRO, CHLORHEXIDINE GLUCONATE, DENTINAL TUBULES, ANTIFUNGAL EFFICACY, NONCULTURABLE STATE, TISSUE DISSOLUTION, MICROORGANISMS, INSTRUMENTATION, SUSCEPTIBILITY
- Ankara University Affiliated: Yes
Abstract
The aim of this study was to evaluate the effectiveness of 2.5 % NaOCl at different temperature and time intervals on Enterococcus faecalis and Candida albicans-infected human roots. A total of 112 root cylinders prepared from extracted single-rooted humans were infected by E. faecalis (Group A, n = 56) or C. albicans (Group B, n = 56); 3 root cylinders served as negative controls. Both groups were further divided into 6 subgroups according to three contact times (30 s, 1 min, 5 min) with NaOCl at two different temperatures (25 or 37 A degrees C). Microorganism growth was controlled at the 24th and 48th hours. Statistical analysis was performed using the Chi-square test. While NaOCl at 25 A degrees C for 5 min was the most effective irrigation regimen to eliminate E. faecalis (p < 0.001), NaOCl at 37 A degrees C for 5 min exhibited significantly superior antifungal properties (p < 0.05). At the same contact times, difference in the temperature of NaOCl did not affect the growth of either E. faecalis or C. albicans. As a result, the irrigation time of NaOCl was more effective than the temperature to eliminate E. faecalis, while pre-heating of NaOCl to 37 A degrees C increased its effectiveness on C. albicans at 5 min contact time.