Immunohistochemical demonstration of p53 protein and metallothioneins in canine mammary tumours

VURAL S., VURAL M. R., KÜPLÜLÜ Ş., Alcigir M., KAYMAZ M.

Revue de Medecine Veterinaire, cilt.160, sa.6, ss.300-307, 2009 (SCI-Expanded) identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 160 Sayı: 6
  • Basım Tarihi: 2009
  • Dergi Adı: Revue de Medecine Veterinaire
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.300-307
  • Anahtar Kelimeler: Dog, Immunohistochemistry, Mammary gland, Metallothionein, P53 protein, Tumour
  • Ankara Üniversitesi Adresli: Evet

Özet

In the study, p53 protein and metallothioneins (MT) were investigated by immunohistochemistry in canine mammary tumours (n=50). These tumours [5 benign mixed tumours (BMT) and 45 malignant tumours whose 36 malignant mixed tumours (MMT) and 9 adenocarcinomas (AC)] mainly involved inguinal (n=18), caudal thoracic (n=11) and caudal abdominal (n=10) glands. Positive p53 protein immunolabelling of nucleus and/or cytoplasm of glandular epithelial cells and more rarely of ductal epithelial cells were observed in all malignant tumours. Furthermore, a moderate to marked p53 staining was evidenced in 75% of MMT and in 44% of AC. Forty-four (98%) malignant tumours also exhibited metallothionein (MT) positive immunostaining of nucleus, cytoplasm or both. The MT positivity was moderate to intense in 82% of positive tumours (30 MMT and 6 AC). The p53 protein and MT expressions with a low to a moderate intensity were detected in 2 and 3 benign mixed tumours respectively and 2 of them simultaneously expressed the 2 markers. These results suggest that the subcellular accumulation of p53 protein and MT is associated with tumour malignancy and that positive MT and p53 protein benign tumours would evolve into malignant tumours.