Immunoblotting analysis of somatic components of Dirofilaria immitis

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Oge H., ÖGE S., YILDIRIM A., Kircali F., KARA M.

PARASITE, cilt.12, sa.2, ss.179-182, 2005 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 12 Sayı: 2
  • Basım Tarihi: 2005
  • Doi Numarası: 10.1051/parasite/2005122179
  • Dergi Adı: PARASITE
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.179-182
  • Anahtar Kelimeler: Dirofilaria immitis, dog, SDS-PAGE, western blot, ANTIGENS, SURFACE
  • Ankara Üniversitesi Adresli: Evet

Özet

SDS-PAGE analysis of Dirofilaria immitis extracts demonstrated the complexity of somatic protein component of adult mole similar to that of adult female worm. Western blot analysis showed six major peptide bands of 85, 66, 42, 20, 16.2 and 14.5 kDa recognized in the sera of infected dogs. Western blotting sera from dogs with Dirofilaria immitis infection suggest that antigenic components in the low molecular weight region may be related to the anti-parasitic mechanism of the host.