Study of programmed cell death in bovine herpesvirus 1 infected MDBK cells and the possible role of nitric oxide in this process

Yazici Z., Baskin Y., Baskin H., Gecer O., Bahar I., ÖZKUL A.

ACTA VETERINARIA HUNGARICA, cilt.52, sa.3, ss.287-297, 2004 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 52 Sayı: 3
  • Basım Tarihi: 2004
  • Doi Numarası: 10.1556/avet.52.2004.3.5
  • Dergi Adı: ACTA VETERINARIA HUNGARICA
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.287-297
  • Anahtar Kelimeler: bovine herpesvirus 1, nitric oxide (NO), programmed cell death, NECROSIS-FACTOR-ALPHA, INDUCED APOPTOSIS, VIRUS-INFECTION, MACROPHAGES, INHIBITION, REPLICATION, INDUCTION, TYPE-1, GAMMA
  • Ankara Üniversitesi Adresli: Evet

Özet

Bovine herpesvirus 1 (BHV-1) is the aetiological agent of many disease types and may predispose infected animals, possibly through immunosuppression, to secondary bacterial infections. Immunosuppression may directly be associated with the induction of programmed cell death (PCD) in some virus-infected cells. Nitric oxide (NO) has an important mediating role against fungal, bacterial, protozoal, viral pathogens and tumours. BHV-1 induced apoptosis between 0.5-3 h postinfection (PI) in MDBK cells; however, between 3 and 6 h PI the PCD response was found to be decreased. It was interesting to see that BHV-1 inhibited staurosporin-induced PCD after 1 h. These results showed similarities with those obtained from herpes simplex type 1 infections in human epithelial cells. PCD response decreased 1 h following caspase-3 inhibitor applications, whereas NO response increased 3 h following infection in the presence of caspase-8 and -9 inhibitory peptides. In conclusion, BHV-1 inhibited the staurosporin-induced apoptotic response and also the NO response. We propose that this inhibition is caspase-3 dependent.