Diagnosis of infectious bronchitis in chickens by polymerase chain reaction and fluorescent antibody technique


AKAN M., Izgur M., Sareyyupolu B.

ANKARA UNIVERSITESI VETERINER FAKULTESI DERGISI, cilt.54, sa.1, ss.47-54, 2007 (SCI-Expanded) identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 54 Sayı: 1
  • Basım Tarihi: 2007
  • Dergi Adı: ANKARA UNIVERSITESI VETERINER FAKULTESI DERGISI
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), TR DİZİN (ULAKBİM)
  • Sayfa Sayıları: ss.47-54
  • Anahtar Kelimeler: chicken, diagnosis, fluorescent antibody technique, infectious bronchitis, polymerase chain reaction, SEROTYPE IDENTIFICATION, IMMUNOFLUORESCENT ASSAY, VIRUS, PCR
  • Ankara Üniversitesi Adresli: Evet

Özet

in the study, a total of 461 materials, 42 of which were from broilers', 18 were from broiler breeders and 11 were from layer flocks, were investigated. In broiler flocks, clinically progressing respiratory: disorders, coughing, sneezing, nasal discharge and in two (4.8%) flocks, at least one of the investigated birds was observed to have affected kidneys. Mortality rate was determined to change amongst flocks between 3-21%. In broiler breeder flocks, a drop by 2-11% in egg production, soft shelled eggs, drop in internal quality of eggs and affected kidneys in 2 (11.1%) flocks accompanying slight respiratory system problems were observed. Mortality rates in these flocks were found to be 3-8% in rearing flocks, 1-4% in laying flocks. In layer flocks, a decline between 6-14% in egg production and a decline in egg shell and internal quality accompanying respiratory distress were investigated. In rearing flocks while the mortalities were changing between 4-11% in layer flocks this ratio was found to change between 1-7%. After RT-PCR tests, IBV was detected in 16.7% of broiler flocks, 22.2% of broiler breeders and 27.3% of layers. IBV was detected from a total number of 14 out of 71 (19.7%) flocks, investigated. A positive reaction in 14.2% of materials obtained from broilers, 16.7% from broiler breeders and 18.2% from layers was observed by FAT. A total number of 11 (15.5%) out of 71 flocks were identified to have IB. According to these results, infectious bronchitis was found to be an important problem concerning chicken production in Turkey. RT-PCR was found to be superior to FAT in the identification of the disease and when performed after passage of suspected materials in embriyonated eggs both assays were observed to be important in determining the positive cases.