Detection of cattle infected with Theileria annulata in fields by nested PCR, IFAT and microscopic examination of blood smears

Vatansever Z., Nalbantoglu S.

TURKISH JOURNAL OF VETERINARY & ANIMAL SCIENCES, cilt.26, sa.6, ss.1465-1469, 2002 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 26 Sayı: 6
  • Basım Tarihi: 2002
  • Dergi Adı: TURKISH JOURNAL OF VETERINARY & ANIMAL SCIENCES
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, TR DİZİN (ULAKBİM)
  • Sayfa Sayıları: ss.1465-1469
  • Anahtar Kelimeler: Theileria annulata, PCR, nested PCR, IFAT, epidemiology, POLYMERASE-CHAIN-REACTION, TICKS, HYBRIDIZATION, EPIDEMIOLOGY
  • Ankara Üniversitesi Adresli: Evet

Özet

The aim of this study was to diagnose Theileria annulata infected cattle by microscopy, IFAT and nested PCR and to compare the sensitivity and specificity of these diagnostic methods. A total of 147 blood and sera samples were collected from healthy cattle in four localities of Polatli district, Ankara, where tropical theileriosis is prevalent. Examination results revealed positivity rates of 31.3%, 44.9% and 61.2% for microscopy, IFAT and nested PCR, respectively. It was shown that nested PCR is more sensitive and specific when compared to microscopical examination and serological findings. It was also shown that nested PCR is more sensitive than single step PCR.