Clonal Micropropagation of Main Grape and Rootstock Varieties of Turkish Viticulture for Obtaining Virus-Free Basic Nursery Stocks

Celik H., SÖYLEMEZOĞLU G., Ertunc F., Cakir A., Dursunoglu S., Akbas B.

9th International Conference on Grape Genetics and Breeding, Udine, İtalya, 31 Mayıs 2009, cilt.827, ss.421-424 identifier identifier

  • Yayın Türü: Bildiri / Tam Metin Bildiri
  • Cilt numarası: 827
  • Doi Numarası: 10.17660/actahortic.2009.827.72
  • Basıldığı Şehir: Udine
  • Basıldığı Ülke: İtalya
  • Sayfa Sayıları: ss.421-424
  • Anahtar Kelimeler: grapevine, virus, meristem culture, basic material
  • Ankara Üniversitesi Adresli: Evet

Özet

The main purpose of this project is establishing the mother blocks with 22 clones of eight grapevine cultivars ('Sultani', 'Kalecik Karasi', 'Cabernet Sauvignon', 'Merlot', 'Shiraz', 'Mourvedre', 'Chardonnay', 'Sauvignon Blanc'), and five clones of five rootstocks ('110 R', '1103 P', '140 Ru', 'Kober 5 BB', '41 B'), which are widely used in different viticultural areas of Turkey. Plant material was tested for eight grape viruses (GFLV, GLRaV-1, 2, 3, 6, GVA, ArMV, GFkV, SLRSV, RpRSV, ToRSV-chi) using DAS-ELISA. Virus-infected clones were in vitro meristem cultured and sub-cultured to eliminate the viruses in MS media and re-tested for the infectious viruses. All previously infected clones were found to be free after in vitro meristem culture. Finally, all clones were mass-propagated by in vitro shoot-tip culture and acclimatized under mist, then transplanted into the plastic containers as a single-node cutting source of the basic nursery stocks.