Metabolome adaptation and oxidative stress response of common carp (Cyprinus carpio) to altered water pollution levels

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Koubova A., Nguyen T. V., Grabicova K., Burkina V., Aydın F. G., Grabic R., ...Daha Fazla

ENVIRONMENTAL POLLUTION, cilt.303, sa. 119117, ss.1-11, 2022 (SCI-Expanded)

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 303 Sayı: 119117
  • Basım Tarihi: 2022
  • Doi Numarası: 10.1016/j.envpol.2022.119117
  • Dergi Adı: ENVIRONMENTAL POLLUTION
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, PASCAL, Aerospace Database, Aqualine, Aquatic Science & Fisheries Abstracts (ASFA), BIOSIS, Biotechnology Research Abstracts, CAB Abstracts, Chimica, Communication Abstracts, Compendex, EMBASE, Environment Index, Food Science & Technology Abstracts, Geobase, Greenfile, MEDLINE, Metadex, Pollution Abstracts, Veterinary Science Database, Civil Engineering Abstracts
  • Sayfa Sayıları: ss.1-11
  • Ankara Üniversitesi Adresli: Evet

Özet

Treated wastewater ponds (TWPs) serve as recipients and passive tertiary treatment mediators for recycled

water. These nutrient-rich habitats are increasingly utilised in aquaculture, nevertheless multiple loads of various

contaminants with adverse effects on aquatic fauna, including fish, have been recorded. In the present study, we

investigated the effects of fish transfer in response to altered levels of pollution on liver metabolic profiles and

tissue-specific oxidative stress biomarkers during short- and long-term exposure. In a field experiment, common

carp (Cyprinus carpio) originating in severely polluted TWP were restocked after one year to a reference pond

with a background pollutant concentration typical of the regional river. In contrast, fish that originated in the

reference pond were restocked to TWP. Fish were sampled 0, 7, 14, 60, and 180 days after restocking and fish

liver, kidney, intestine, and gill tissues were subjected to biomarker analysis. Pharmaceutically active compounds

(PhACs) and metabolic profiles were determined in fish liver using liquid chromatography high-resolution mass

spectrometry (LC-HRMS). Fish transferred from reference to polluted pond increased the antioxidant response

and absorbed PhACs into metabolism within seven days. Fish liver metabolic profiles were shifted rapidly, but

after 180 days to a lesser extent than profiles in fish already adapted in polluted water. Restocked fish from

polluted to reference pond eliminated PhACs during the short phase within 14 days, and the highest antioxidant

response accompanied the depuration process. Numerous elevated metabolic compounds persisted in such

exposed fish for at least 60 days. The period of two weeks was suggested as sufficient for PhACs depuration, but

more than two months after restocking is needed for fish to stabilise their metabolism. This study contributed to

determining the safe handling with marketed fish commonly restocked to wastewaters and clarified that water

pollution irreversibly altered fish metabolic profile