Akademik Veri Yönetim Sistemi
INTERNATIONAL JOURNAL OF PHARMACEUTICS, cilt.332, sa.1-2, ss.161-167, 2007 (SCI-Expanded)
Meso-tetraphenyl porphine (mTPP) is a highly lipophilic, florescent porphyrin derivate and it is used as photosensitizer on the treatment of malign neoplasms. The aim of this study was to prepare mTPP loaded pluronic F127 and polyethylene glycol-distearoyl phosphatidylethanolamine (PEG(2000)-DSPE) micelles to evaluate polymeric micelles potential for the transport of drugs through intestinal mucosa. Transport and bioadhesion behaviors of polymeric micelles was investigated using Caco-2 cell monolayer and everted rat intestine models. In order to show that Caco-2 cells can be used as a transport model cytotoxicity of formulations was tested. Cell viability was more than 80%, showing that Caco-2 cells will keep their viability during the transport studies demonstrating that prepared formulations can be securely used as oral drug carrier systems. Plain micelles were labeled with a fluorescent agent rhodamine-phosphatidylethanolamine (Rh-PE)and their transport through Caco-2 cells was investigated beside mTPP loaded micelles. At the end of 4 h transport study through Caco-2 cells, cumulative transport (%) of fluorescent agents were around 14% and 1% in Rh-PE labeled and mTPP loaded micelles This difference was attributed to the different placement of mTPP and Rh-PE in the micellar core. Drug transport was not estimated in everted rat intestine model but the bioadhesion was 79% and 70% for mTPP loaded pluronic F 127 and PEG2000-DSPE micelles. These good bioadhesion rates are promising for oral drug delivery. (c) 2006 Elsevier B.V. All rights reserved.