Electrochemical DNA biosensor based on poly(2,6-pyridinedicarboxylic acid) modified glassy carbon electrode for the determination of anticancer drug gemcitabine

AYDOĞDU TIĞ G., ZEYBEK B., Pekyardimci S.

TALANTA, cilt.154, ss.312-321, 2016 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 154
  • Basım Tarihi: 2016
  • Doi Numarası: 10.1016/j.talanta.2016.03.049
  • Dergi Adı: TALANTA
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.312-321
  • Anahtar Kelimeler: Gemcitabine, dsDNA, Glassy carbon electrode, Polymer, Differential pulse voltammetry, PENCIL GRAPHITE ELECTRODE, STRIPPING VOLTAMMETRIC DETERMINATION, PAT GENE FRAGMENT, CALF THYMUS DNA, 2,6-PYRIDINEDICARBOXYLIC ACID, PASTE ELECTRODE, URIC-ACID, POLYMER, COMPLEXES, PLASMA
  • Ankara Üniversitesi Adresli: Evet

Özet

In this study, a simple methodology was used to develop a new electrochemical DNA biosensor based on poly(2,6-pyridinedicarboxylic acid) (P(PDCA)) modified glassy carbon electrode (GCE). This modified electrode was used to monitor for the electrochemical interaction between the dsDNA and gemcitabine (GEM) for the first time. A decrease in oxidation signals of guanine after the interaction of the dsDNA with the GEM was used as an indicator for the selective determination of the GEM via differential pulse voltammetry (DPV). The guanine oxidation peak currents were linearly proportional to the concentrations of the GEM in the range of 1-30 mg L-1. Limit of detection (LOD) and limit of quantification (LOQ) were found to be 0.276 mg L-1 and 0.922 mg L-1, respectively. The reproducibility, repeatability, and applicability of the analysis to pharmaceutical dosage forms and human serum samples were also examined. In addition to DPV method, UV-vis and viscosity measurements were utilized to propose the interaction mechanism between the GEM and the dsDNA. The novel DNA biosensor could serve for sensitive, accurate and rapid determination of the GEM. (c) 2016 Elsevier B.V. All rights reserved.