Fabrication of amperometric cholesterol biosensor based on SnO2 nanoparticles and Nafion-modified carbon paste electrode

AYDOĞDU TIĞ G., KOYUNCU ZEYBEK D., Pekyardimci S.

CHEMICAL PAPERS, cilt.70, sa.6, ss.695-705, 2016 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 70 Sayı: 6
  • Basım Tarihi: 2016
  • Doi Numarası: 10.1515/chempap-2016-0005
  • Dergi Adı: CHEMICAL PAPERS
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.695-705
  • Anahtar Kelimeler: cholesterol biosensor, carbon paste electrode, amperometry, SnO2 nanoparticles, HYDROGEN-PEROXIDE BIOSENSOR, FLOW-INJECTION ANALYSIS, TIN OXIDE, URIC-ACID, REAGENTLESS BIOSENSOR, ZNO NANOPARTICLES, COMPOSITE FILM, ASCORBIC-ACID, NANOTUBES, GRAPHENE
  • Ankara Üniversitesi Adresli: Evet

Özet

This study reports the fabrication of an amperometric cholesterol biosensor based on cholesterol oxidase (ChOx), SnO(2)NPs and Nafion-modified carbon paste enzyme electrodes (CPE/SnO(2)NPs-ChOx/Naf). The electrochemical characterisations of BCPE and CPE/SnO(2)NPs were performed using CV and EIS. The determination of cholesterol was carried out by electrochemical oxidation of H2O2 at 0.6 V vs. Ag/AgCl. The CPE/SnO(2)NPs-ChOx/Naf presented a linear range from 0.20 mu mol L-1 to 4.95 mu mol L-1 with a low limit of detection (0.04 mu mol L-1). In addition, the optimal values for pH and temperature were found to be 7.5 and 35 degrees C, respectively. The CPE/SnO(2)NPs-ChOx/Naf was used for the determination of cholesterol in serum samples and good results were obtained. (C) 2016 Institute of Chemistry, Slovak Academy of Sciences