Akademik Veri Yönetim Sistemi
JOURNAL OF FOOD AGRICULTURE & ENVIRONMENT, cilt.10, sa.2, ss.130-132, 2012 (SCI-Expanded)
In this study, the presence of Listeria monocytogenes in cig kofte without meat was analysed. Seventy samples were purchased from various markets and retail centres in Ankara over an 8-month period. Bacterial colonies suspected to be L. monocytogenes were isolated by a method that begins with a primary enrichment in Half-Fraser broth followed by immunomagnetic separation (IMS) with magnetic beads coated with an antibody against L. monocytogenes and plating on PALCAM and Oxford agars. Subsequent selective identification on Harlequin (TM) Listeria Medium and polymerase chain reaction (PCR) confirmation were used. The PCR method used a primer pair targeted at a specific region of the internalin (inlA) gene of L. monocytogenes, giving a product of 250 bp. Detection of L. monocytogenes in the 70 samples with IMS and PCR primers specific to the inlA gene that encodes the virulence of L. monocytogenes showed that 17.1% (12 of 70) samples contained L. monocytogenes. The inlA gene was detected in 12 L. monocytogenes isolates obtained from different cig kofte samples. The data gathered in this study are important for the assessment of the risk of listeriosis posed by L. monocytogenes in cig kofte, even when it is prepared without meat. Consumers should be warned of the health risks associated with eating such raw foods to protect the public from listeriosis.