Exploring the Molecular Basis of Anticancer Activity in Various Mushroom Species Against Colorectal Cancer Cells

Ceylan I., Ozerkan D., Emin N., Akata I., Altuner E. M.

LETTERS IN DRUG DESIGN AND DISCOVERY, vol.22, no.18, pp.1-8, 2024 (SCI-Expanded, Scopus)

  • Publication Type: Article / Article
  • Volume: 22 Issue: 18
  • Publication Date: 2024
  • Doi Number: 10.2174/0115701808324049241108063414
  • Journal Name: LETTERS IN DRUG DESIGN AND DISCOVERY
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Biotechnology Research Abstracts, Chemical Abstracts Core
  • Page Numbers: pp.1-8
  • Ankara University Affiliated: Yes

Abstract

Background

Mushrooms are shown to protect against the side effects of cancer. Therefore, mushrooms with proven anticancer properties and active ingredients are fascinating in the search for new cancer drugs.

Objective

In this study, the effects of extracts from Hericium coralloides (M1), Lactarius deliciosus (M2), Lepista nuda (M3), Pleurotus ostreatus (M4), and Suillus collitinus (M5) together on HCT116 were investigated. Mesenchymal stem cells (MSCs) were used to study the effect on healthy cells.

Methods

MTT was used to determine cell viability. Dose-response curves were generated, the IC50 values of the compounds were calculated, and the effect of the extracts was compared using it. The FTIR was used to analyze the quantitative changes of the cellular components.

Results and Discussion

The evaluation of the IC50 values of all fungal species showed that they reduced the cell viability of HCT116 cells. In contrast, no significant reduction in cell viability was observed in MSCs. Changes in the ratio of cell membrane lipids, proteins, and cell nucleic acids between control and fungal-treated HCT116 cells were detected by FTIR. Many of the chemotherapeutic agents are of plant origin, and many resources should still be explored to inhibit the side effects of cancer therapy.

Conclusion

The data obtained through this experiment will serve as a reference for studies to be a new source of anticancer drugs in modern pharmacology.