Untangling a Gordian knot: the role of a GluCl3 I321T mutation in abamectin resistance in Tetranychus urticae

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Xue W., Mermans C., Papapostolou K., Lamprousi M., Christou I., İNAK E., ...More

PEST MANAGEMENT SCIENCE, vol.77, no.4, pp.1581-1593, 2021 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 77 Issue: 4
  • Publication Date: 2021
  • Doi Number: 10.1002/ps.6215
  • Journal Name: PEST MANAGEMENT SCIENCE
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, PASCAL, Periodicals Index Online, Agricultural & Environmental Science Database, Aqualine, Aquatic Science & Fisheries Abstracts (ASFA), BIOSIS, CAB Abstracts, Chemical Abstracts Core, Chimica, EMBASE, Environment Index, Food Science & Technology Abstracts, Geobase, MEDLINE, Pollution Abstracts, Veterinary Science Database
  • Page Numbers: pp.1581-1593
  • Keywords: avermectin, ivermectin, arthropoda, GluCl, Rdl, two&#8208, electrode voltage&#8208, clamp electrophysiology, GATED CHLORIDE CHANNEL, 2-SPOTTED SPIDER-MITE, INSECTICIDE RESISTANCE, TARGET-SITE, ACARICIDE RESISTANCE, TRANSMEMBRANE REGION, PLUTELLA-XYLOSTELLA, POINT MUTATION, AVERMECTINS, ACID
  • Ankara University Affiliated: Yes

Abstract

BACKGROUND The cys-loop ligand-gated ion channels, including the glutamate-gated chloride channel (GluCl) and GABA-gated chloride channel (Rdl) are important targets for drugs and pesticides. The macrocyclic lactone abamectin primarily targets GluCl and is commonly used to control the spider mite Tetranychus urticae, an economically important crop pest. However, abamectin resistance has been reported for multiple T. urticae populations worldwide, and in several cases was associated with the mutations G314D in GluCl1 and G326E in GluCl3. Recently, an additional I321T mutation in GluCl3 was identified in several abamectin resistant T. urticae field populations. Here, we aim to functionally validate this mutation and determine its phenotypic strength.