Investigation of Brucella antibodies in bovine sera by rose bengal plate test (RBPT), serum agglutination test (SAT), microagglutination test (MAT) and 2-mercaptoethanol-microagglutination (2-ME-MAT) test

SAREYYÜPOĞLU B., Cantekin Z., MÜŞTAK H. K.

ANKARA UNIVERSITESI VETERINER FAKULTESI DERGISI, cilt.57, sa.3, ss.157-160, 2010 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 57 Sayı: 3
  • Basım Tarihi: 2010
  • Doi Numarası: 10.1501/vetfak_0000002368
  • Dergi Adı: ANKARA UNIVERSITESI VETERINER FAKULTESI DERGISI
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, TR DİZİN (ULAKBİM)
  • Sayfa Sayıları: ss.157-160
  • Anahtar Kelimeler: Brucella, microagglutination, serology, serum agglutination, DIAGNOSIS
  • Ankara Üniversitesi Adresli: Evet

Özet

In this study, for the investigation of Brucella antibodies a total number of 524 serum samples were sent to the Department of Microbiology, Faculty of Veterinary Medicine, Ankara University. For the determination of most appropriate test for the investigation, first 100 samples were investigated by rose bengal plate test (RBPT), serum agglutination test (SAT), microagglutination test (MAT) and 2-mercaptoethanol-microagglutination test (2-ME-MAT). Following this pre-investigation study, 3 (3%) and 5 (5%) samples were found to be positive with RBPT and SAT, MAT, 2-ME-MAT (same results were achieved with each of the three tests) tests, respectively. No statistically significant difference was observed amongst the later three tests (p>0.05). Because it has a short evaluation time and since it was found to be economical concerning the used expenditure and reagents, MAT was preferred as the most appropriate test for the serological investigation. Following MAT tests of 524 serum samples 8 (1.5%) samples were found to be positive concerning Brucella antibodies. As a conclusion, MAT was determined to be a fast, reliable and an economic test, which can be easily used in routine diagnostic laboratories for serological diagnosis.