IMMUNOHISTOCHEMICAL VISUALIZATION OF THE ENTERIC NERVOUS-SYSTEM USING ANTIBODIES AGAINST PROTEIN GENE-PRODUCT (PGP) 9.5

KRAMMER H., KARAHAN S. T., RUMPEL E., KLINGER M., KUHNEL W.

ANNALS OF ANATOMY-ANATOMISCHER ANZEIGER, cilt.175, sa.4, ss.321-325, 1993 (SCI-Expanded, Scopus) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 175 Sayı: 4
  • Basım Tarihi: 1993
  • Doi Numarası: 10.1016/s0940-9602(11)80029-4
  • Dergi Adı: ANNALS OF ANATOMY-ANATOMISCHER ANZEIGER
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.321-325
  • Anahtar Kelimeler: PGP 95, ENTERIC NERVOUS SYSTEM, AUTONOMIC NERVOUS SYSTEM, IMMUNOHISTOCHEMISTRY, PORCINE SMALL-INTESTINE, NEURON-SPECIFIC ENOLASE, PIG, ELECTROPHORESIS, S-100
  • Ankara Üniversitesi Adresli: Evet

Özet

Both the investigation of the enteric nervous system and the diagnosis of its pathological changes require reliable staining methods. In order to assess the potential of protein gene product (PGP) 9.5 as a marker for the enteric nervous system, we examined its immunoreaction in whole-mount preparations of the guinea pig and porcine small intestine, using a rabbit polyclonal antiserum.The immunohistochemical technique reveals the fundamental architectural features of the ganglionic and aganglionic plexuses. Furthermore, it enables a reproducible and differentiated visualization of the enteric nerve cells to be made, so that the various nerve cell types can be morphologically identified.