Association of the tumor necrosis factor-alpha-308 G/A polymorphism with nasal polyposis

Batikhan H., GÖKCAN M. K., Beder E., Akar N., Ozturk A., Gerceker M.

EUROPEAN ARCHIVES OF OTO-RHINO-LARYNGOLOGY, cilt.267, sa.6, ss.903-908, 2010 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 267 Sayı: 6
  • Basım Tarihi: 2010
  • Doi Numarası: 10.1007/s00405-009-1167-5
  • Dergi Adı: EUROPEAN ARCHIVES OF OTO-RHINO-LARYNGOLOGY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.903-908
  • Anahtar Kelimeler: Nasal polyps, Tumor necrosis factor-alpha, Polymorphism/single nucleotide, Polymerase chain reaction, Case-control study, Mutation, FACTOR-ALPHA PROMOTER, TNF-ALPHA, GENE POLYMORPHISMS, CHRONIC RHINOSINUSITIS, ASTHMA, SUSCEPTIBILITY
  • Ankara Üniversitesi Adresli: Evet

Özet

Nasal polyposis (NP) is a chronic inflammatory disease in which several molecular and cellular interactions play important roles. Tumor necrosis factor-alpha (TNF-alpha) is a major pro-inflammatory cytokine with a key role in immune inflammatory responses in NP. Altered levels of TNF-alpha, which may occur due to polymorphisms in the TNF-alpha promoter region, may also be associated with NP susceptibility. Given these facts, we investigated the possible association of the TNF-alpha -308 G/A single nucleotide polymorphism (SNP) with NP. In this study, 97 consecutive adult patients with NP and 95 age- and gender-matched controls were recruited. For identification of SNP, restriction fragment length polymorphism analysis after polymerase chain reaction was carried out. The NP group had a significantly higher rate of polymorphism compared to controls (p = 0.015). Logistic regression analysis revealed that the presence of the TNF-alpha -308 G/A SNP is an independent risk factor for NP development (OR, 3.68; CI, 1.27-10.7; p = 0.016). The presence of a mutation failed to influence disease severity on the basis of resistance to medical and/or surgical treatment. This study suggests a possible linkage of a SNP in the TNF-alpha promoter with NP. These results need to be confirmed with multicentre studies for more precise interpretation and corroborative studies for investigating the influence of polymorphism on transcriptional activity.