High frequency adventitious shoot regeneration in sainfoin

Ozgen M., Ozcan S., Sevimay C. S., SANCAK C., Yildiz M.

PLANT CELL TISSUE AND ORGAN CULTURE, sa.3, ss.205-208, 1998 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Basım Tarihi: 1998
  • Doi Numarası: 10.1023/a:1006097714968
  • Dergi Adı: PLANT CELL TISSUE AND ORGAN CULTURE
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.205-208
  • Anahtar Kelimeler: Onobrychis viciifolia, organogenesis, PLANT-REGENERATION, ONOBRYCHIS-VICIIFOLIA, PROTOPLASTS, CULTURE, SCOP
  • Ankara Üniversitesi Adresli: Evet

Özet

A procedure is described for the rapid and efficient adventitious shoot regeneration from leaflets, petioles and stems of field-grown sainfoin plants. All explants formed shoots on a range of media supplemented with 6-benzyladenine (BA) and alpha-naphthaleneacetic acid (NAA). Stem explants appeared to have better regeneration capacity than leaflet and petiole explants in most media tested. The highest frequency of shoot regeneration was achieved from stem segments on a medium containing 20 mu M BA and 0.5 mu M NAA. Regenerated shoots rooted in half-strength Murashige and Skoog medium containing 5 mu M indole-3-butyric acid and later established well under greenhouse conditions.