Medically important Candida spp. identification: an era beyond traditional methods

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EVREN E., Gocmen J. S., Hortac Istar E., Yavuzdemir S., Tekeli A., YAVUZ Y., ...Daha Fazla

TURKISH JOURNAL OF MEDICAL SCIENCES, cilt.52, sa.3, ss.834-840, 2022 (SCI-Expanded) identifier identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 52 Sayı: 3
  • Basım Tarihi: 2022
  • Doi Numarası: 10.55730/1300-0144.5380
  • Dergi Adı: TURKISH JOURNAL OF MEDICAL SCIENCES
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, CAB Abstracts, EMBASE, MEDLINE, Veterinary Science Database, TR DİZİN (ULAKBİM)
  • Sayfa Sayıları: ss.834-840
  • Anahtar Kelimeler: Candida identification, MALDI-TOF MS, CHROMagar Candida, corn meal tween-80 agar, sequencing, DESORPTION IONIZATION-TIME, FLIGHT MASS-SPECTROMETRY, YEAST IDENTIFICATION, CHROMAGAR CANDIDA, ALBICANS, SYSTEMS
  • Ankara Üniversitesi Adresli: Evet

Özet

Background/aim: Candida infections are gaining more attention for the last few decades so diagnostic tools are very important for early diagnosis. Conventional identification of yeasts is time-consuming, molecular methods are more complicated and relatively expensive gold-standard methods. Matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF MS) was put into the market due to its speed and high accuracy. The aim of this study was to evaluate the performance of corn meal tween-80 agar (CMTA), CHROMagar Candida medium, and MALDI-TOF MS and to compare the obtained results with DNA sequencing. Materials and methods: The CHROMagar Candida medium, CMTA, and MALDI-TOF MS Biotyper System were used to test 416 isolates. The isolates with discrepant results by at least one of the three methods were subjected to sequence analysis. Results: The identification results of the 351 (%84.4) were compatible with all three methods. When compared to the sequencing results, the most accurate results were obtained by the MALDI-TOF MS, especially for rare Candida species. Conclusion: MALDI-TOF MS is found to be the most accurate identification tool for clinically important Candida strains. CMTA alone should not be used for the final identification of Candida species and the chromogenic medium should always be considered presumptive.