Optimizing Ultrasonic-Assisted Extraction Process of Paralepista flaccida: A Comparative Study of Antioxidant, Anticholinesterase, and Antiproliferative Activities via Response SurfaceMethodology and Artificial Neural NetworkModeling

Sevindik M., Gürgen A., Korkmaz A. F., Akata I.

MOLECULES, cilt.30, ss.1-17, 2025 (SCI-Expanded)

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 30
  • Basım Tarihi: 2025
  • Dergi Adı: MOLECULES
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Aerospace Database, Biotechnology Research Abstracts, CAB Abstracts, Chemical Abstracts Core, Communication Abstracts, Food Science & Technology Abstracts, MEDLINE, Metadex, Veterinary Science Database, Directory of Open Access Journals, Civil Engineering Abstracts
  • Sayfa Sayıları: ss.1-17
  • Ankara Üniversitesi Adresli: Evet

Özet

In this study, extraction conditions were optimized to maximize the biological activities of

extracts obtained from Paralepista flaccida, an edible mushroom species. Extraction processes

were carried out using an ultrasonically assisted system, and two different optimization approaches

were used as follows: Response Surface Methodology (RSM) and Artificial Neural

Network–Genetic Algorithm (ANN-GA). The antioxidant potentials of the optimized extracts

were evaluated using DPPH, FRAP, TAS, TOS, and OSI parameters; anticholinesterase

activities were measured against AChE and BChE enzymes; and antiproliferative activities

were investigated in A549, MCF-7, and DU-145 human cancer cell lines. In addition,

phenolic contents were determined by LC-MS/MS analysis. The findings revealed that the

extracts obtained by the RSM method exhibited a superior biological profile compared to

ANN-GA extracts in terms of antioxidant, anticholinesterase, and antiproliferative activities.

The high cytotoxicity observed, particularly in the MCF-7 line, supports the anticancer

potential of this extract. These results demonstrate that optimization strategies are crucial

for increasing not only extract yield but also biological functionality.