Voltammetric studies on the HIV-1 inhibitory drug Efavirenz: The interaction between dsDNA and drug using electrochemical DNA biosensor and adsorptive stripping voltammetric determination on disposable pencil graphite electrode

DOĞAN TOPAL B., USLU B., ÖZKAN S. A.

BIOSENSORS & BIOELECTRONICS, cilt.24, sa.8, ss.2358-2364, 2009 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 24 Sayı: 8
  • Basım Tarihi: 2009
  • Doi Numarası: 10.1016/j.bios.2008.12.005
  • Dergi Adı: BIOSENSORS & BIOELECTRONICS
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.2358-2364
  • Anahtar Kelimeler: Efavirenz, dsDNA, Biosensor, Drugs, Determination, Adsorptive stripping voltammetry, REVERSE-TRANSCRIPTASE INHIBITOR, PERFORMANCE LIQUID-CHROMATOGRAPHY, HUMAN PLASMA, MITOMYCIN-C, PHARMACEUTICALS, ABACAVIR, GUANINE, AGENT
  • Ankara Üniversitesi Adresli: Evet

Özet

The interaction of Efavirenz (EFV) with fish sperm dsDNA immobilized onto pencil graphite electrode (PGE) has been studied by using differential pulse voltammetric technique using an electrochemical DNA biosensor. The guanine signal was lower with (double stranded-DNA) dsDNA-treated PGE than the untreated electrode after the interaction with EFV occurred. The changes in the experimental parameters such as the accumulation time and the concentration of EFV were also studied. All necessary parameters such as sensitivity, selectivity, accuracy and precision were calculated. In addition, the detection and determination limits, reproducibility and applicability of the analysis to pharmaceutical dosage forms were also investigated. These results showed that this DNA biosensor could be used for the sensitive, rapid simple and cost effective detection and determination of EFV-dsDNA interaction. The linearity was between 2 and 24 ppm of EFV concentration on guanine signal decreasing curve.