Electrochemical xanthine biosensor based on zinc oxide nanoparticles‒ multiwalled carbon nanotubes‒1,4-benzoquinone composite

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Dalkıran B., KAÇAR SELVİ C., Erden P. E., Kılıç E.

Journal of the Turkish Chemical Society, Section A: Chemistry, cilt.5, sa.1, ss.317-332, 2018 (Scopus) identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 5 Sayı: 1
  • Basım Tarihi: 2018
  • Doi Numarası: 10.18596/jotcsa.307414
  • Dergi Adı: Journal of the Turkish Chemical Society, Section A: Chemistry
  • Derginin Tarandığı İndeksler: Scopus, TR DİZİN (ULAKBİM)
  • Sayfa Sayıları: ss.317-332
  • Anahtar Kelimeler: Biosensor, Carbon nanotubes, Mediator, Xanthine, Zinc oxide nanoparticles
  • Ankara Üniversitesi Adresli: Evet

Özet

© 2018, Turkish Chemical Society. All rights reserved.Zinc oxide nanoparticles (ZnONPs), multiwalled carbon nanotubes (MWCNTs) and 1,4-benzoquinone (BQ) dispersed in chitosan (CS) matrix were used to construct a xanthine biosensor. Xanthine oxidase (XOx) was immobilized onto BQ-MWCNTs-ZnO–CS composite modified glassy carbon electrode (GCE) using glutaraldehyde as the crosslinking agent. The parameters of the construction process and the experimental variables for the biosensor were optimized. The xanthine biosensor showed optimum response within 10 s, and the sensitivity was 39.4 μA/mMcm2 at +0.25 V (vs. Ag/AgCl). The linear working range of the biosensor was found to be 9.0×10−71.1×10−4 M with a detection limit of 2.1×10-7 M. The biosensor exhibited good long-term stability and reproducibility. The presented biosensor was also used for monitoring the freshnesses of chicken and beef flesh.