TDZ x IBA induced shoot regeneration from cotyledonary leaves and in vitro multiplication in safflower (Carthamus tinctorius L.)

BAŞALMA D., Uranbey S., Mirici S., Kolsarici O.

AFRICAN JOURNAL OF BIOTECHNOLOGY, cilt.7, sa.8, ss.960-966, 2008 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 7 Sayı: 8
  • Basım Tarihi: 2008
  • Dergi Adı: AFRICAN JOURNAL OF BIOTECHNOLOGY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.960-966
  • Anahtar Kelimeler: Carthamus tinctorius, regeneration, cotyledonary leaves, HIGH-FREQUENCY INDUCTION, SOMATIC EMBRYOGENESIS, PLANT-REGENERATION, HYPOCOTYL SEGMENTS, THIDIAZURON, MICROPROPAGATION
  • Ankara Üniversitesi Adresli: Evet

Özet

A high frequency adventitious shoot regeneration protocol for safflower (Carthamus tinctorius L.) cv. Din er with high yield (Turkish cultivar) using cotyledonary leaves of 10 day-old seedlings were optimized by studying the influence of different combinations of thidiazuron (TDZ) and indole-3-butyric acid (IBA). Cotyledonary leaves were cultured on Murashige and Skoog medium (MS) supplemented with different concentrations and combinations of TDZ and IBA. The highest percentage of regenerated shoots (33.33%) and the highest number of shoots per explant (6.5) occurred on a MS medium containing 0.5 mg/l TDZ and 0.25 mg/l IBA. Furthermore, cotyledonary nodes and meristem tips of 14 day-old seedlings were cultured on Murashige and Skoog (MS) medium supplemented with various concentrations of N-6-benzylamino-purine (BAP) alone or combination of -naphthalene acetic acid (NAA). Direct multiple shoots from cotyledonary nodes and meristem tips developed within 18 - 21 days in most media tested. 100% shoot multiplication was achieved from cotyledonary node and meristem tip on a range of MS media supplemented with different concentrations of BAP and NAA.