Osteogenic differentiation of encapsulated rat mesenchymal stem cells inside a rotating microgravity bioreactor: in vitro and in vivo evaluation

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KOÇ DEMİR A., ELÇİN A. E., ELÇİN Y. M.

CYTOTECHNOLOGY, cilt.70, sa.5, ss.1375-1388, 2018 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 70 Sayı: 5
  • Basım Tarihi: 2018
  • Doi Numarası: 10.1007/s10616-018-0230-8
  • Dergi Adı: CYTOTECHNOLOGY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.1375-1388
  • Anahtar Kelimeler: Cell encapsulation, Mesenchymal stem cells, Composite microbeads, Osteogenic differentiation, Microgravity bioreactor, Bone tissue engineering, CHITOSAN-HYDROXYAPATITE MICROSPHERES, COMPOSITE MICROSPHERES, BONE REGENERATION, NANOFIBERS, ALGINATE, COLLAGEN, GROWTH, REPAIR
  • Ankara Üniversitesi Adresli: Evet

Özet

The objective of this study is to evaluate the in vitro and in vivo osteogenic potential of rat bone marrow mesenchymal stem cells (BM-MSCs) using chitosan/hydroxyapatite (C/HAp) microbeads as encapsulation matrix under osteoinductive medium and dynamic culture conditions. The degradation characteristics of C/HAp microbeads were evaluated under in vitro and in vivo conditions for 180days. BM-MSCs were encapsulated in C/HAp microbeads with >85% viability, and were cultured in a slow turning lateral vessel-type rotating bioreactor simulating microgravity conditions for 28days, under the effect of osteogenic inducers. MTT assay showed that the metabolic activity of encapsulated cells was preserved >80% after a week. In vitro experiments confirmed that the encapsulated BM-MSCs differentiated into osteoblastic cells, formed bone-like tissue under osteogenic microgravity bioreactor conditions. Preliminary in vivo study indicated C/HAp microbeads containing BM-MSCs were able to repair the surgically-created small bone defects in the rat femur. BM-MSCs-C/HAp composite microbeads may have potential for modular bone regeneration.