VALIDATION STUDY OF A SPECTROPHOTOMETRIC BINARY MIXTURE ANALYSIS BİR SPEKTROFOTOMETRİK İKİLİ KARIŞIM ANALİZİNİN DOĞRULAMA ÇALIŞMASI

ÜSTÜNDAĞ Ö., DİNÇ E.

Ankara Universitesi Eczacilik Fakultesi Dergisi, cilt.45, sa.3, ss.545-553, 2021 (Scopus) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 45 Sayı: 3
  • Basım Tarihi: 2021
  • Doi Numarası: 10.33483/jfpau.989819
  • Dergi Adı: Ankara Universitesi Eczacilik Fakultesi Dergisi
  • Derginin Tarandığı İndeksler: Scopus, Central & Eastern European Academic Source (CEEAS), EMBASE, TR DİZİN (ULAKBİM)
  • Sayfa Sayıları: ss.545-553
  • Anahtar Kelimeler: Binary mixture, Quantitative determination, Signal processing, Validation
  • Ankara Üniversitesi Adresli: Evet

Özet

Objective: A rapid spectrophotometric analysis by applying a signal processing method was validated by intra-day and inter-day experiments, standard addition technique, recovery study and analysis of variance (ANOVA). For this purpose, a binary pharmaceutical formulation was evaluated consisting of candesartan cilexetil (CC) and hydrochlorothiazide (HT). Material and Method: The absorption spectra of mixtures and tablet solutions in the spectral range 200-305 nm were measured by a Shimadzu UV-1601 dual-beam UV-VIS spectrophotometer with a constant gap width (2 nm). Result and Discussion: Intra-day and inter-day experiments, standard addition technique, recovery study and analysis of variance (ANOVA) studies were carried out by using artificial mixtures were prepared for this purpose. These studies have shown that the validity and applicability of the method is good.