Investigation of antioxidant and cytotoxic activities of Opuntia ficus-indica (L.) Mill. fruit extract

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Okur M. E., Karakas N., Karadag A. E., Uludag D., Polat D.

ISTANBUL JOURNAL OF PHARMACY, cilt.49, sa.3, ss.154-160, 2019 (ESCI)

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 49 Sayı: 3
  • Basım Tarihi: 2019
  • Doi Numarası: 10.26650/istanbuljpharm.2019.19035
  • Dergi Adı: ISTANBUL JOURNAL OF PHARMACY
  • Derginin Tarandığı İndeksler: Emerging Sources Citation Index (ESCI)
  • Sayfa Sayıları: ss.154-160
  • Anahtar Kelimeler: Extract, Opuntia ficus-indica, brain cancer cell line, colon cancer cell line, antioxidant, PHENOLIC COMPOSITION, NATURAL-PRODUCTS, IN-VITRO, QUERCETIN, CANCER, CHEMOTHERAPY, FLAVONOIDS, MYRICETIN, BETANIN, PARTS
  • Açık Arşiv Koleksiyonu: AVESİS Açık Erişim Koleksiyonu
  • Ankara Üniversitesi Adresli: Evet

Özet

Opuntia ficus-indica fruits are claimed to possess several health-beneficial properties. This study was carried out to determine the phytochemicals as well as to compare antioxidant activity and cell viability of the methanol extract of OFI-fruits. Antioxidant activity was evaluated by various in vitro tests such as DPPH and ABTS. LC-MS and HPLC analyses were then performed. The activity of the OFI-fruit extract on viability of U87-MG (glioblastoma multiform), HT-29 (colon cancer) and human primary fibroblast cells was investigated by determining viable cells on a luciferase based system (Cell TiterGlo; Promega; USA). The results showed that OFI-fruits were a source of antioxidant compounds. Moreover, the extract was found to have rich phenolic-flavonoid contents. Quercetin was found in the extract according to LC-MS and HPLC studies. A remarkable increased cell death in the HT-29 and U87 cell lines was detected in a concentration-dependent manner (1-10mg/mL). Among these cell lines, HT-29 responded to OFI-fruit treatment more sensitively to even the lowest dose (1mg/mL). The results of this study showed that the OFI-fruit has significant antioxidant capacity and leads to strong cytotoxic activity on various cancer cells. Therefore, it can be suggested that OFI-fruits may have chemo-preventive potential and can be studied further to clarify cytotoxic abilities.