Determination of lamivudine and zidovudine in binary mixtures using first derivative spectrophotometric, first derivative of the ratio-spectra and high-performance liquid chromatography-UV methods

Uslu B., Ozkan S. A.

Analytica Chimica Acta, cilt.466, sa.1, ss.175-185, 2002 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 466 Sayı: 1
  • Basım Tarihi: 2002
  • Doi Numarası: 10.1016/s0003-2670(02)00545-7
  • Dergi Adı: Analytica Chimica Acta
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.175-185
  • Anahtar Kelimeler: lamivudine, zidovudine, first derivative spectrophotometry, ratio derivative spectrophotometry, HPLC, pharmaceuticals, human serum, TANDEM MASS-SPECTROMETRY, HUMAN PLASMA, BLOOD-CELLS, HUMAN SERUM, HYDROCHLOROTHIAZIDE, QUANTIFICATION, TABLETS, HPLC, 3'-AZIDO-3'-DEOXYTHYMIDINE, GLUCURONIDE
  • Ankara Üniversitesi Adresli: Evet

Özet

Three methods are presented for the simultaneous determination of lamivudine and zidovudine. The first method depends on first derivative UV spectrophotometry, with zero-crossing and peak-to-base measurement. The first derivative amplitudes at 265.6 and 271.6nm were selected for the assay of lamivudine and zidovudine, respectively. The second method depends on first derivative of the ratio-spectra by measurements of the amplitudes at 239.5 and 245.3nm for lamivudine and 225.1 and 251.5nm for zidovudine. Calibration graphs were established for 1-50μg/ml for lamivudine and 2-100μg/ml for zidovudine. In the third method (HPLC), a reversed-phase column with a mobile phase of methanol:water:acetonitrile (70:20:10 (v/v/v)) at 0.9ml/min flow rate was used to separate both compounds with a detection of 265.0nm. Linearity was obtained in the concentration range of 0.025-50μg/ml for lamivudine and 0.15-50μg/ml for zidovudine. All of the proposed methods have been extensively validated. These methods allow a number of cost and time saving benefits. The described methods can be readily utilized for analysis of pharmaceutical formulations. There was no significant difference between the performance of all of the proposed methods regarding the mean values and standard deviations. The described HPLC method showed to be appropriate for simultaneous determination of lamivudine and zidovudine in human serum samples. © 2002 Elsevier Science B.V. All rights reserved.