Akademik Veri Yönetim Sistemi
Microchemical Journal, cilt.221, 2026 (SCI-Expanded, Scopus)
Zanamivir (ZAN) is utilized in the treatment of infections caused by both influenza A and influenza B viruses. It acts as a novel inhibitor of the enzyme neuraminidase, which is a surface glycoprotein crucial for the replication of influenza viruses. In this study, we present the first molecularly imprinted polymer (MIP)–based electrochemical sensor designed for the selective and sensitive detection of ZAN. The sensor was fabricated using the photopolymerization (PP) technique on the surface of a glassy carbon electrode (GCE), employing 4-aminobenzoic acid (4-ABA) as the functional monomer and ZAN as the template molecule. Under optimized experimental conditions, the 4-ABA/ZAN@MIP-GCE sensor exhibited a linear range of 3.75 × 10−12–3.75 × 10−11 M, achieving detection and quantification limits of 3.06 × 10−13 M and 1.02 × 10−12 M in standard solutions. Electrochemical characterization was conducted using cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), and differential pulse voltammetry (DPV), utilizing a 5.0 mM [Fe(CN)6]3−/4− solution as a redox probe for the quantitative detection of ZAN. The developed sensor was also characterized through scanning electron microscopy (SEM) measurements. The recovery values for the MIP-based sensors were determined to be 100.73% for commercial serum samples and 101.23% for urine samples. Additionally, the relative selectivity coefficient (k') of the proposed sensor was calculated. This newly designed sensor offers a promising approach for the rapid, sensitive, economical, and selective analysis of ZAN. The electronic and molecular changes on the electrode surface were evaluated using quantum chemical calculations. Furthermore, a Green Analytical Chemistry (GAC) assessment categorized the sensor as an environmentally superior alternative to conventional methods. Consequently, the suggested sensors exhibited remarkable selectivity, elevated sensitivity, consistent repeatability, and reproducibility in their performance for ZAN analysis in both commercial serum and urine samples.