BIOCATALYSIS AND BIOTRANSFORMATION, cilt.35, sa.6, ss.407-416, 2017 (SCI-Expanded)
Production of methyl gallate (MG), which is an important phenolic acid ester for pharmaceutical industry, was carried out by Novozym 435-catalysed transesterification of propyl gallate (PG) with methanol in a deep eutectic solvent. Reaction parameters governing substrate molar ratio, enzyme concentration, temperature and agitation rate were investigated batch-wise in choline chloride: glycerol-water binary mixture. The results were evaluated in terms of conversion of PG, yield of MG and hydrolysis of PG to gallic acid. 10% (w/w) of water was found to be favourable in the reaction medium for low hydrolysis percent. The highest conversion (17.4%) and yield (60.4%) but the lowest hydrolysis (2%) after 120 h of transesterification were found at PG/methanol molar ratio of 1:6, enzyme concentration of 40 g/L, 50 degrees C and 200 rpm. A kinetic model based on the Ping-Pong Bi-Bi mechanism for transesterification of PG was proposed with the assumption that there were no internal and external mass transfer resistances.