Expression patterns of genes encoding proteins with peritrophin A domains and protein localization in Mamestra configurata

TOPRAK U., Baldwin D., Erlandson M., Gillott C., Harris S., Hegedus D. D.

JOURNAL OF INSECT PHYSIOLOGY, cilt.56, sa.12, ss.1711-1720, 2010 (SCI-Expanded, Scopus) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 56 Sayı: 12
  • Basım Tarihi: 2010
  • Doi Numarası: 10.1016/j.jinsphys.2010.06.016
  • Dergi Adı: JOURNAL OF INSECT PHYSIOLOGY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.1711-1720
  • Anahtar Kelimeler: Peritrophin A domain, Peritrophic matrix, Chitin binding, Gene expression, Protein localization, MOSQUITO ANOPHELES-GAMBIAE, CHITIN-BINDING PROTEINS, MATRIX-PROTEIN, INTESTINAL MUCIN, PROTEOMIC ANALYSIS, LUCILIA-CUPRINA, AEDES-AEGYPTI, IDENTIFICATION, CLONING, MIDGUT
  • Ankara Üniversitesi Adresli: Hayır

Özet

Genes encoding three proteins (McPPAD1-3) with peritrophin A chitin-binding domains (PADs) were identified from a Mamestra configurata larval midgut cDNA library In addition to midgut McPPAD1-3 and a previously identified gene encoding the peritrophin McPM1 were expressed in foregut hindgut Malpighian tubules tracheae fat body and cuticle however the corresponding McPPAD proteins exhibited different localization patterns McPPAD1 was restricted to the digestive tract and Malpighian tubules McPPAD2 to Malpighian tubules and McPPAD3 to the foregut midgut hindgut tracheae and cuticle Protein fold recognition analysis using tachycitin as a guide structure modelled the McPPAD1 PADs but not McPPAD2 or McPPAD3 PADs The McPPAD1 PADs were predicted to contain three antiparallel beta-sheets and a hevein-like fold that form a chitin-binding pocket containing two hydrophobic R-groups in a sandwich-like orientation (C) 2010 Elsevier Ltd All rights reserved