Ectopic osteogenic tissue formation by MC3T3-E1 cell-laden chitosan/hydroxyapatite composite scaffold

Koc A., Elçin A. E., Elçin Y. M.

Artificial Cells, Nanomedicine and Biotechnology, vol.44, no.6, pp.1440-1447, 2016 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 44 Issue: 6
  • Publication Date: 2016
  • Doi Number: 10.3109/21691401.2015.1036998
  • Journal Name: Artificial Cells, Nanomedicine and Biotechnology
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.1440-1447
  • Keywords: bone tissue engineering, biocompatibility, chitosan/hydroxyapatite, composite scaffold, ectopic bone formation, preosteoblast, xenotransplantation, ENDOTHELIAL GROWTH-FACTOR, IN-VITRO DIFFERENTIATION, STEM-CELLS, BONE, CHITOSAN, HYDROXYAPATITE, OSTEOBLAST, INDUCTION, RESPONSES, ADHESION
  • Ankara University Affiliated: Yes

Abstract

© 2015 Informa Healthcare USA, Inc.This study evaluates the suitability of a macroporous three-dimensional chitosan/hydroxyapatite (CS/HA) composite as a bone tissue engineering scaffold using MC3T3-E1 cells. The CS/HA scaffold was produced by freeze-drying, and characterized by means of SEM and FTIR. In vitro findings demonstrated that CS/HA supported attachment and proliferation of cells, and stimulated extracellular matrix (ECM) production. Tissue biocompatibility and osteogenic capacity of the cell-laden constructs were evaluated in an ectopic Wistar rat model. In vivo results showed that the MC3T3-E1 cell-laden CS/HA was essentially histocompatible, promoted neovascularization and calcified matrix formation, and secreted osteoblast-specific protein. We conclude that the composite scaffold evaluated has potential for applications in bone regeneration.