A novel QCM immunosensor development based on gold nanoparticles functionalized sulfur-doped graphene quantum dot and h-ZnS-CdS NC for Interleukin-6 detection

Atar N., Yola M. L.

ANALYTICA CHIMICA ACTA, cilt.1148, 2021 (SCI-Expanded, Scopus) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 1148
  • Basım Tarihi: 2021
  • Doi Numarası: 10.1016/j.aca.2021.338202
  • Dergi Adı: ANALYTICA CHIMICA ACTA
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Aerospace Database, Aqualine, Aquatic Science & Fisheries Abstracts (ASFA), Artic & Antarctic Regions, BIOSIS, Biotechnology Research Abstracts, CAB Abstracts, Chemical Abstracts Core, Chimica, Communication Abstracts, Compendex, EMBASE, Food Science & Technology Abstracts, MEDLINE, Metadex, Pollution Abstracts, Veterinary Science Database, Civil Engineering Abstracts
  • Ankara Üniversitesi Adresli: Hayır

Özet

Interleukin 6 (IL-6) acts as both a proinflammatory and anti-inflammatory cytokine and is generally utilized as an important diagnostic biomarker for sepsis. In addition, the high levels of IL-6 measured in plasma have been associated with pathological inflammation. A novel quartz crystal microbalance (QCM) immunoassay method was presented for high sensitivity and selectivity detection of interleukin-6 (IL-6) based on gold nanoparticles functionalized sulfur-doped graphene quantum dot (AuNPs/S-GQD) and hollow ZnS-CdS nanocage (h-ZnS-CdS NC). Firstly, AuNPs/S-GQD nanocomposite was synthesized in the presence of tetrachloroauric acid and then conjugated onto anti-IL-6 antibodies by amino-gold affinity. The sandwich-type QCM immunoassay probe was prepared by immune-reaction between AuNPs/S-GQD/QCM immobilized with anti-IL-6 capture antibodies and h-ZnS-CdS NC including detection antiIL-6 antibodies in the presence of target IL-6. The prepared QCM immunoassay probe was characterized by transmission electron microscopy (TEM), scanning electron microscope (SEM), x-ray diffraction (XRD) method, x-ray photoelectron spectroscopy (XPS), Raman spectroscopy, UV-vis spectroscopy, Fourier transform infrared spectroscopy (FTIR), cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). The QCM immunosensor showed a linearity range (0.01-2.0 pg mL(-1) ) and a low detection limit (3.33 fg mL(-1)). Lastly, high stable and selective QCM immunosensor was applied to prepared plasma samples with good recovery. (C) 2021 Elsevier B.V. All rights reserved.