Differentiation of benign and malignant regions in paraffin embedded tissue blocks of pulmonary adenocarcinoma using micro CT scanning of paraffin tissue blocks: a pilot study for method validation

Kayı Cangır A., Sak S., Gunes G., Orhan K.

SURGERY TODAY, vol.51, no.10, pp.1594-1601, 2021 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 51 Issue: 10
  • Publication Date: 2021
  • Doi Number: 10.1007/s00595-021-02252-2
  • Journal Name: SURGERY TODAY
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, CAB Abstracts, EMBASE, MEDLINE, Veterinary Science Database
  • Page Numbers: pp.1594-1601
  • Keywords: Micro-CT, Lung cancer, Diagnostic tool, Histopathology
  • Ankara University Affiliated: Yes

Abstract

Purpose Micro computed tomography (micro-CT) can provide detailed information about the internal structure of materials. This study aimed to demonstrate the diagnostic value of micro-CT in formalin fixed paraffin embedded pulmonary adenocarcinomas by correlating the micro-CT findings of tumoral and non-tumoral areas with hematoxylin and eosin (HE) sections. Methods Paraffin blocks obtained from three adenocarcinomas were scanned with micro-CT. Ten regions of interest (ROIs) from adenocarcinoma and 11 ROIs from pulmonary parenchyma (ROI-C and ROI-N, respectively) areas were compared regarding the various structural parameters. Results All parameters were significantly different regarding the tumoral and non-tumoral ROIs. The percent object volume, structure thickness, structure linear density, connectivity and connectivity density were higher in ROI-Cs (p < 0.000, p < 0.000, p = 0.001, p < 0.000, and p < 0.000 respectively); whereas intersection surface and structure model index were higher in ROI-Ns (p < 0.000 and p < 0.000). The open porosity percentage was higher in ROI-Ns (68.86 + 2.96 vs 48.29 + 5.11, p < 0.000) and the closed porosity percentage was higher in ROI-Cs (2.29 + 0.55 vs 0.57 + 0.17 p < 0.000). Conclusions The tumoral and non-tumoral areas in paraffin blocks can be distinguished from each other, using the quantitative and qualitative information obtained by micro-CT. Making this distinction with quantitative data obtained from micro-CT can therefore be the basis of creating artificial intelligence algorithms in the future.