Akademik Veri Yönetim Sistemi
2022 Current Trends in Forensic Toxicology Symposium, 16 - 20 Mayıs 2022, ss.1
Opioid use disorder (OUD) and alcohol use disorder (AUD) is a chronic recurrent health problem that affects millions of people each year [1]. Naltrexone (NTX) is an opioid antagonist that has been shown to reduce opioid use and craving, and also reduce alcohol craving and alcohol use in patients with alcohol dependence. Naltrexone has highly efficacious blocking capability with competitive antagonist activity at µ-opioid receptors. The presented study aims to develop a dispersive liquid-liquid microextraction method for more sensitive, reliable, and greener detection and quantification of NTX and its main metabolite 6β-Naltrexol (6βNTX) by using high-performance liquid chromatography (HPLC) followed by UV detection. Because of the necessity of sensitivity for analysis, new approaches are needed in sample preparation techniques.
The motivation behind this study is based on the literature survey which reveals to there are not any dispersive liquid-liquid microextraction techniques used. In the literature, mostly used techniques were liquid-liquid extraction and solid-phase extraction before the analysis of these substances. During the development of the extraction method, Acetonitrile, acetone, and methanol were compared as dispersive solvents, chloroform, chlorobenzene, carbon tetrachloride, carbon disulfide, 1,2 dichloroethane, and trichloroethylene were compared as extraction solvents. In addition, the effects of ionic strength and pH on extraction were investigated.
For the separation and the determination of these compounds, different mobile phase systems, buffer types, buffer concentrations, flow rates, stationary phase chemistries, and temperatures were optimized for the development of an efficient HPLC-UV method. As an analytical column, Kinetex EVO C18 (150 mm x 4.6 mm i.d., 2.6 μm) (Phenomenex, Torrance, CA) was used for the separation of the NTX and 6βNTX. Phosphate buffer (pH: 2.5) and methanol (80:20, v/v) at a flow rate of 0.4 mL/min were found as optimum conditions.
The optimized method was validated according to the International Council for Harmonization Guidelines, and the applicability of the method was demonstrated using commercial plasma samples.