Akademik Veri Yönetim Sistemi
Australian Journal of Crop Science, cilt.8, sa.7, ss.1019-1023, 2014 (SCI-Expanded)
This study was aimed to determine the effect of in vitro spacing competition among explants for water and for both macro and micro nutrients in the growth medium on tissue culture response of Lathyrus chrysanthus Boiss. cotyledon node explants. Cotyledon node explants were excised from 14-day-old sterile seedlings and cultured in a petri dish at '0.5 × 0.5', '1.0 × 1.0' and '2.0 × 2.0' cm distance for 4 weeks. At the end of the culture, fresh and dry weights of explant, water content of explant, shoot regeneration percentage, shoot number per explant, shoot length, total shoot number per petri dish and total chlorophyll content in leaves of regenerated shoots were recorded. The positive effect of spacing competition among explants was observed in all characters examined at '1.0 × 1.0' cm culture distance. Shoot number per cotyledon node explant and total shoot number per petri dish were obtained the highest as 2.99 and 30.67, respectively, at '1.0 × 1.0' cm culture distance. According to the results, it could be concluded that encouraging explants for spacing competition by decreasing the culture distances among them from '2.0 × 2.0' cm to '1.0 × 1.0' cm caused to a significant increases in all characters examined. This study showed that the spacing competition among explants under in vitro conditions could be evaluated to increase the success of tissue culture studies for related genotype.