Electrochemical Determination and in silico Studies of Fludarabine on NH2 Functionalized Multiwalled Carbon Nanotube Modified Glassy Carbon Electrode
Electroanalysis, cilt.32, sa.1, ss.37-49, 2020 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 32 Sayı: 1
- Basım Tarihi: 2020
- Doi Numarası: 10.1002/elan.201900347
- Dergi Adı: Electroanalysis
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Aerospace Database, Applied Science & Technology Source, BIOSIS, Chimica, Communication Abstracts, Metadex, Civil Engineering Abstracts
- Sayfa Sayıları: ss.37-49
- Anahtar Kelimeler: Fludarabine, nanosensor, multi walled carbon nanotubes, thermodynamic, molecular dynamics, MOLECULAR-DYNAMICS, ADENINE, GUANINE, TRIPHOSPHATE, QUANTIFICATION, IDENTIFICATION, NANOSENSORS, VOLTAMMETRY, OXIDATION, DOCKING
- Ankara Üniversitesi Adresli: Evet
Özet
© 2019 Wiley-VCH Verlag GmbH & Co. KGaA, WeinheimA sensitive voltammetric technique has been developed for the determination of Fludarabine using amine-functionalized multi walled carbon nanotubes modified glassy carbon electrode (NH2-MWCNTs/GCE). Molecular dynamics simulations, an in silico technique, were employed to examine the properties including chemical differences of Fludarabine- functionalized MWCNT complexes. The redox behavior of Fludarabine was examined by cyclic, differential pulse and square wave voltammetry in a wide pH range. Cyclic voltammetric investigations emphasized that Fludarabine is irreversibly oxidized at the NH2-MWCNTs/GCE. The electrochemical behavior of Fludarabine was also studied by cyclic voltammetry to evaluate both the kinetic (ks and Ea) and thermodynamic (ΔH, ΔG and ΔS) parameters on NH2-MWCNTs/GCE at several temperatures. The mixed diffusion-adsorption controlled electrochemical oxidation of Fludarabine revealed by studies at different scan rates. The experimental parameters, such as pulse amplitude, frequency, deposition potential optimized for square-wave voltammetry. Under optimum conditions in phosphate buffer (pH 2.0), a linear calibration curve was obtained in the range of 2×10−7 M–4×10−6 M solution using adsorptive stripping square wave voltammetry. The limit of detection and limit of quantification were calculated 2.9×10−8 M and 9.68×10−8 M, respectively. The developed method was applied to the simple and rapid determination of Fludarabine from pharmaceutical formulations.