Determination of phenytoin in exhaled breath condensate using electromembrane extraction followed by capillary electrophoresis

Seyfinejad B., Meshkini A., Habibolahi P., Ozkan S. A., Jouyban A.

Electrophoresis, cilt.41, sa.9, ss.666-677, 2020 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 41 Sayı: 9
  • Basım Tarihi: 2020
  • Doi Numarası: 10.1002/elps.201900440
  • Dergi Adı: Electrophoresis
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Aerospace Database, BIOSIS, Biotechnology Research Abstracts, CAB Abstracts, Chimica, Communication Abstracts, EMBASE, Food Science & Technology Abstracts, MEDLINE, Metadex, Veterinary Science Database, Civil Engineering Abstracts
  • Sayfa Sayıları: ss.666-677
  • Anahtar Kelimeler: Capillary electrophoresis, Central composite design, Electromembrane extraction, Exhaled breath condensate, Phenytoin, SOLID-PHASE EXTRACTION, PERFORMANCE LIQUID-CHROMATOGRAPHY, ANTIEPILEPTIC DRUGS, HUMAN PLASMA, PACKED SORBENT, MICROEXTRACTION, LAMOTRIGINE, CARBAMAZEPINE, SAMPLES, ASSAY
  • Ankara Üniversitesi Adresli: Evet

Özet

© 2020 WILEY-VCH Verlag GmbH & Co. KGaA, WeinheimApplication of hollow fiber-based electromembrane extraction was studied for extraction and quantification of phenytoin from exhaled breath condensate (EBC). Phenytoin is extracted from EBC through a supported liquid membrane consisting of 1-octanol impregnated in the walls of a hollow fiber, and into an alkaline aqueous acceptor solution inside the lumen of the fiber. Under the obtained conditions of electromembrane extraction, that is, the extraction time of 15 min, stirring speed of 750 rpm, donor phase pH at 11.0, acceptor pH at 13.0, and an applied voltage of 15 V across the supported liquid membrane, an enrichment factor of 102-fold correspond to extraction percent of 25.5% was achieved. Good linearity was obtained over the concentration range of 0.001–0.10 µg/mL (r2 = 0.9992). Limits of detection and quantitation were 0.001 and 0.003 µg/mL, respectively. The proposed method was successfully applied to determine phenytoin from EBC samples of patients receiving the drug. No interfering peaks were detected that indicating excellent selectivity of the method. The intra- and interday precisions (RSDs) were less than 14%.