The determination of beta-agonist residues in bovine tissues using liquid chromatography-tandem mass spectrometry

Yikilmaz Y., Kuzukiran O., Erdogan E., Sen F., Kirmizibayrak O., FİLAZİ A.

BIOMEDICAL CHROMATOGRAPHY, cilt.34, sa.10, 2020 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 34 Sayı: 10
  • Basım Tarihi: 2020
  • Doi Numarası: 10.1002/bmc.4926
  • Dergi Adı: BIOMEDICAL CHROMATOGRAPHY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, BIOSIS, Biotechnology Research Abstracts, CAB Abstracts, Chemical Abstracts Core, Chimica, EMBASE, MEDLINE, Veterinary Science Database
  • Anahtar Kelimeler: Beta-agonist, kidney, LC-MS, MS, liver, muscle, MEAT-PRODUCTS, LIVER, CLENBUTEROL, RACTOPAMINE, QUANTIFICATION, URINE, VALIDATION, ZILPATEROL, QUECHERS, ASSAY
  • Ankara Üniversitesi Adresli: Evet

Özet

We aimed to develop a rapid, simple and reproducible method based on LC-tandem mass spectrometry (LC-MS/MS) to analyze beta-agonist residues (clenbuterol, zilpaterol, ractopamine and isoxsuprine) in bovine tissues. The method was validated in accordance with the European Council Decision 2002/657/EC. The samples were homogenized, and then 10 mL of an acetate buffer was added to a 5-g sample. The sample was then centrifuged at 12,000 rpm and filtered. Sodium hydroxide (2m) was added to adjust pH of the sample that was centrifuged again. The extract was filtered through a solid-phase extraction column. The residue was re-dissolved in 250 mu L acetonitrile and then subjected to LC-MS/MS. The separation was done on a C(18)column. The mobile phase consisted of 0.1% formic acid in deionized water and 0.1% formic acid in methanol. The mean recoveries of beta-agonists were in the range of 84.3%-119.1% with relative standard deviations (%RSDs) of 0.683%-4.05%. Decision limits and detection capabilities of the analytes ranged from 0.0960 to 4.9349 mu g/kg and from 0.0983 to 5.0715, respectively. This method was used to detect four beta-agonists in 100 bovine muscle, 100 liver and 100 kidney tissues from a slaughterhouse. No residue was found above the maximum residue limit level.