Batch and continuous hydrolysis of lactose using beta-galactosidase immobilized on gelatin-CMC

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Atıf İçin Kopyala

Sungur S., Yildirim Ö.

POLYMER-PLASTICS TECHNOLOGY AND ENGINEERING, cilt.38, sa.4, ss.821-829, 1999 (SCI-Expanded)

• Yayın Türü: Makale / Tam Makale
• Cilt numarası: 38 Sayı: 4
• Basım Tarihi: 1999
• Doi Numarası: 10.1080/03602559909351616
• Dergi Adı: POLYMER-PLASTICS TECHNOLOGY AND ENGINEERING
• Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
• Sayfa Sayıları: ss.821-829
• Ankara Üniversitesi Adresli: Evet

Özet

beta-Galactosidase enzyme (E,C. 3.2.1.23) was immobilized onto a gelatin-carboxymethylcellulose carrier system by using cross-linker chromium acetate, A relative activity increase of 33% was achieved by adding carboxymethylcellulose to gelatin, The effect of operation temperature and pH on relative activity and reusability of the immobilized enzyme was investigated, A fluidized-bed reactor with a downflow mechanism was used for continuous process hydrolysis, and a constant glucose production rate was obtained.