Batch and continuous hydrolysis of lactose using beta-galactosidase immobilized on gelatin-CMC
POLYMER-PLASTICS TECHNOLOGY AND ENGINEERING, cilt.38, sa.4, ss.821-829, 1999 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 38 Sayı: 4
- Basım Tarihi: 1999
- Doi Numarası: 10.1080/03602559909351616
- Dergi Adı: POLYMER-PLASTICS TECHNOLOGY AND ENGINEERING
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
- Sayfa Sayıları: ss.821-829
- Ankara Üniversitesi Adresli: Evet
Özet
beta-Galactosidase enzyme (E,C. 3.2.1.23) was immobilized onto a gelatin-carboxymethylcellulose carrier system by using cross-linker chromium acetate, A relative activity increase of 33% was achieved by adding carboxymethylcellulose to gelatin, The effect of operation temperature and pH on relative activity and reusability of the immobilized enzyme was investigated, A fluidized-bed reactor with a downflow mechanism was used for continuous process hydrolysis, and a constant glucose production rate was obtained.