The Combined Effect of the Organic Modifier Content and pH of the Mobile Phase on the Chromatographic Behavior of Some Arylpropionic and Arylacetic Acids to Optimize Their Liquid Chromatographic Determinations

Secilmis-Canbay H., ÇUBUK DEMİRALAY E., Alsancak G., ÖZKAN S. A.

CHROMATOGRAPHIA, cilt.75, sa.13-14, ss.711-720, 2012 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 75 Sayı: 13-14
  • Basım Tarihi: 2012
  • Doi Numarası: 10.1007/s10337-012-2255-z
  • Dergi Adı: CHROMATOGRAPHIA
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.711-720
  • Anahtar Kelimeler: Column liquid chromatography, Combined effect, NSAID, Method validation, Retention factor, PHARMACEUTICAL DOSAGE FORMS, REFERENCE VALUE STANDARDS, IONIZABLE COMPOUNDS, SOLVENT COMPOSITION, ANTIINFLAMMATORY DRUGS, MODELING RETENTION, WATER, HPLC, FORMULATIONS, TEMPERATURE
  • Ankara Üniversitesi Adresli: Evet

Özet

The equation that express the combined effect of the organic modifier content and mobile phase pH has been used to predict the chromatographic behavior of flurbiprofen, ibuprofen, diclofenic acid, ketoprofen, fenoprofen, naproxen, bezafibric acid and clofibric acid. Correlations between the retention factors of the solutes and pH of the mobile phase were determined from a set of 40 chromatograms for each compound at four different levels of acetonitrile composition (40, 45, 50 and 55 %, v/v), and at ten pH values in each acetonitrile-water mixtures. In this way, a complete description of the retention behavior of each solute in the space defined by the pH and organic modifier percentages variables was obtained. The optimized chromatographic condition was applied to quantitative determination of flurbiprofen, ibuprofen, and diclofenic acid in pharmaceutical dosage forms.