Immunohistochemical localization and expression of heat shock proteins (HSP27, HSP60, HSP70, and HSP90) during the oestrous cycle, pregnancy, and lactation in rat ovaries

Kandil B., Kurtdede N., Bayraktaroğlu A. G.

Acta Histochemica, vol.126, no.3, 2024 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Review
  • Volume: 126 Issue: 3
  • Publication Date: 2024
  • Doi Number: 10.1016/j.acthis.2024.152157
  • Journal Name: Acta Histochemica
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Arts and Humanities Citation Index (AHCI), Scopus, Academic Search Premier, Aerospace Database, Communication Abstracts, EMBASE, Metadex, Civil Engineering Abstracts
  • Keywords: Follicle cell, Heat shock protein, Immunohistochemistry, Interstitial cell, Luteal cell, Rat
  • Ankara University Affiliated: Yes


This study aimed to determine the expressions of HSP27, HSP60, HSP70, and HSP90 in rat ovaries during the oestrous cycle, pregnancy, and lactation. In follicle cells, HSP27 and HSP70 expression was not observed. HSP60 in oocytes was higher in the early stages of follicular development but decreased and disappeared as the follicle grew. HSP60 in granulosa and theca cells increased with follicle development and decreased with atresia. HSP90 in follicle cells did not change during follicle development or atresia. The expression of HSPs in interstitial cells was higher in the proestrus and estrus phases of the estrous cycle. The expression of HSPs in these cells was higher on day 5 of pregnancy, decreased on day 10, and decreased further on days 15 and 20. The expression of HSPs, which decreased in the second half of pregnancy, increased again on the first day of lactation. The expression of HSPs then decreased on day 5 of lactation and further decreased on days 10 and 20. HSP60 and HSP90 were positive in new and old corpus luteums (CLs) and their expression did not change during luteal development or regression. HSP27 and HSP70 were absent in new CLs. HSP27 was positive in old CLs and showed the same staining pattern during luteal regression. HSP70 expression was determined in old cyclic CLs during the oestrous cycle and pregnancy and decreased with luteal regression. HSP70 expression in old pregnancy CLs during lactation was very weak compared to the oestrous cycle and pregnancy. In conclusion, HSP60 and HSP90 may participate in folliculogenesis, luteal development, and steroidogenesis in luteal cells, and HSP27, HSP60, HSP70, and HSP90 may be effective in luteal regression and steroidogenesis in interstitial cells. HSP27 and HSP70 may be used as markers to identify old CLs in rats.