Akademik Veri Yönetim Sistemi
35th Anniversary World Congress on Biosensors, Lisbon, Portekiz, 19 - 22 Mayıs 2025, (Yayınlanmadı)
Design of bacteriophage-gated silica sensor for bacteria detection
Extraintestinal diseases caused by Escherichia coli, a Gram-negative bacterium, primarily include urinary tract infections, followed by nosocomial pneumonia, cholecystitis, peritonitis, osteomyelitis, sepsis, and neonatal meningitis. E. coli is frequently isolated from patients with sepsis and is responsible for over 80% of urinary tract infections and a significant proportion of hospital-acquired infections. Current clinical detection methods for E. coli typically require 24–48 hours, emphasizing the need for rapid, sensitive, affordable, and easy-to-use detection methods suitable for on-site analysis (Pakbin et al., 2021). Bacteriophages (phages) are viruses that specifically infect bacteria by recognizing unique receptors, making them valuable tools for bacterial detection. Their specificity and ease of isolation from environmental samples have made phages widely used in sensor development (Çağatay, 2023).
Gated sensors are systems in which dyes are entrapped within the voids of a porous material, held in place by a supramolecular or biomolecular gate. These materials release the encapsulated dye in the presence of a target molecule, generating a measurable optical signal. Since a single analyte can trigger the release of multiple dye molecules, gated sensors offer high sensitivity (Aznar et al., 2016).
In this study, we present a fast, simple, and cost-effective method for detecting E.coli using bacteriophage-gated silica particles. The sensory material releases fluorescent dyes in E. coli- containing samples in under five minutes. Several parameters were optimized, and the sensor was successfully tested on real water samples collected from three sewage sites and Sapanca Lake in Türkiye.