Comparison of renal lesions in cats and dogs using pathomorphological and immunohistochemical methods

Kutlu T., Alcigir G.

BIOTECHNIC & HISTOCHEMISTRY, vol.94, no.2, pp.126-133, 2019 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 94 Issue: 2
  • Publication Date: 2019
  • Doi Number: 10.1080/10520295.2018.1522670
  • Journal Name: BIOTECHNIC & HISTOCHEMISTRY
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.126-133
  • Keywords: Chronic disease, epithelial-mesenchymal transformation, fibrosis, kidney, nephritis, nephrosis, EPITHELIAL-MYOFIBROBLAST TRANSDIFFERENTIATION, PROGRESSIVE TUBULOINTERSTITIAL FIBROSIS, MESENCHYMAL TRANSITION EMT, NESTIN EXPRESSION, TUBULAR CELLS, KIDNEY, VIMENTIN, FAILURE, IDENTIFICATION, INJURY
  • Ankara University Affiliated: Yes

Abstract

We investigated the pathogenesis of chronic renal fibrosis in cats and dogs using immunohistochemistry. We used the avidin-biotin complex peroxidase (ABC-P) method with antibodies against transforming growth factor-beta 1, cytokeratin, E-cadherin, S100A4, alpha-smooth muscle actin, vimentin and nestin to determine whether tubule epithelial cells had undergone epithelial-mesenchymal transformation (EMT) that resulted in loss of epithelial cells and an increased number of mesenchymal cells. Although nephrosis was more common in dogs, nephritis was more common in cats; these pathologies developed in both kidneys. We found that EMT participated in the pathogenesis of renal fibrosis in both dogs and cats.