DETERMINATION OF SERTRALINE AND ITS METABOLITE BY HIGH-PRESSURE LIQUID CHROMATOGRAPHY IN PLASMA
REVUE ROUMAINE DE CHIMIE, cilt.60, sa.5-6, ss.543-548, 2015 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 60 Sayı: 5-6
- Basım Tarihi: 2015
- Dergi Adı: REVUE ROUMAINE DE CHIMIE
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
- Sayfa Sayıları: ss.543-548
- Ankara Üniversitesi Adresli: Evet
Özet
A fast, simple and sensitive high-pressure liquid chromatography (HPLC) method with UV detection was developed for frequently prescribed antidepressant, sertraline (SERT) and its main metabolite N-desmethylsertraline (DSERT), in human plasma. SERT and DSERT were extracted by an optimized solid phase chromatographic (SPE) method using C-18 cartridges and Clomipramine was used as external standard (ES). The analytes were separated on C18, 4.6 mm x 150 mm, 5 pm column at 50 degrees C with a mobile phase of 45% acetonitrile + 55% NaH2PO4 at a flow rate of 0.4 mL/min. Detector responses monitored at 4 different wave-lengths; 200-205-210-215 nm. The method proved to be rapid and effective for the plasma sample analyses of therapeutic drug monitoring for sertraline treated patients.