Determination of Linoleate Isomerase Enzyme Activity of Anaerobic Rumen Fungus Caecomyces sp. GMLF12

Comlekcioglu U., Ozkose E., Kar B., Akyol İ., Ekinci M. S.

KAHRAMANMARAS SUTCU IMAM UNIVERSITY JOURNAL OF NATURAL SCIENCES, vol.14, no.4, pp.12-17, 2011 (ESCI) identifier

  • Publication Type: Article / Article
  • Volume: 14 Issue: 4
  • Publication Date: 2011
  • Journal Name: KAHRAMANMARAS SUTCU IMAM UNIVERSITY JOURNAL OF NATURAL SCIENCES
  • Journal Indexes: Emerging Sources Citation Index (ESCI)
  • Page Numbers: pp.12-17
  • Keywords: Rumen, Caecomyces, Biohidrogenation, Linoleate isomerase, Conjugated Linoleic Acid
  • Ankara University Affiliated: No

Abstract

In this study, the presence of linoleate isomerase enzyme was investigated in Caecomyces sp. GMLF12. Linoleate isomerase enzyme enables the isomerisation of linoleic acid to conjugated linoleic acid. Caecomyces sp. GMLF12 isolate was inoculated to different concentrations of linoleic acid-containing media, but the fungi were not developed. Upon this, Caecomyces sp. GMLF12 was grown in glucose containing medium and spherical rhizoids were used as enzyme source. Cells were treated with linoleic acid, and enzymatic reaction was halted at given time intervals and CLA levels were measured by spectrophotometrically. The maximum CLA level was measured as 8.22 mu g/ml at 45. min. The results showed that Caecomyces sp. GMLF12 have linoleate isomerase enzyme and this enzyme was found in cell surface.