Spectrophotometric quantitative determination of cilazapril and hydrochlorothiazide in tablets by chemometric methods

DİNÇ E., Baleanu D.

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, cilt.30, sa.3, ss.715-723, 2002 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 30 Sayı: 3
  • Basım Tarihi: 2002
  • Doi Numarası: 10.1016/s0731-7085(02)00359-x
  • Dergi Adı: JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.715-723
  • Anahtar Kelimeler: chemometric methods, quantitative determination, cilazapril, hydrochlorothiazide, tablets, PERFORMANCE LIQUID-CHROMATOGRAPHY, DERIVATIVE UV SPECTROPHOTOMETRY, BENAZEPRIL HYDROCHLORIDE, LEAST-SQUARES, PHARMACEUTICAL FORMULATIONS, AMILORIDE HYDROCHLORIDE, ENALAPRIL MALEATE, SPIRONOLACTONE, COMBINATION, CAPTOPRIL
  • Ankara Üniversitesi Adresli: Hayır

Özet

Four chemometric methods were applied to simultaneous determination of cilazapril and hydrochlorothiazide in tablets. Classical least-square (CLS), inverse least-square (ILS), principal component regression (PCR) and partial least-squares (PLS) methods do not need any priori graphical treatment of the overlapping spectra of two drugs in a mixture. For all chemometric calibrations a concentration set of the random mixture consisting of the two drugs in 0.1 M HCl and methanol (1:1) was prepared. The absorbance data in the UV-Vis spectra were measured for the 15 wavelength points (from 222 to 276 nm) in the spectral region 210-290 nm considering the intervals of Deltalambda = 4 nm. The calibration of the investigated methods involves only absorbance and concentration data matrices. The developed calibrations were tested for the synthetic mixtures consisting of two drugs and using the Maple V software the chemometric, calculations were performed. The results of the methods were compared each other as well as with HPLC method and a good agreement was found. (C) 2002 Elsevier Science B.V. All rights reserved.